Otome Yōkai Zakuro (おとめ妖怪ざくろ, "Maiden Spirit Zakuro") is a Japanese manga series written and illustrated by Lily Hoshino. It began serialization in November 2006 in the monthly seinen manga magazine Comic Birz. The first tankōbon volume was released by Gentosha in January 2008; as of March 2015, ten volumes have been released. An anime adaptation produced by J.C.Staff and directed by Chiaki Kon airing in Japan from October to December 2010.

The author announced in October 2016 that she was putting all her manga on hiatus to take care of her newborn son. Hoshino added she hopes to return to her regular work as soon as possible.

During the Westernization of an alternate Japan, humans and spirits coexist. To maintain harmony between the two, the Ministry of Spirits is established and humans and spirit representatives are chosen. Lieutenants Kei Agemaki, Riken Yoshinokazura, and Ganryu Hanakiri are chosen to be the human representatives and are partnered with Zakuro, Susukihotaru, and Bonbori and Hozuki, respectively. However, Zakuro cannot stand humans who accept Jesuit practices so easily and Agemaki has a severe fear of spirits.

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