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This is a List of lesbian-themed fiction. It includes books from the 18th century through the 21st century. It also includes lists of works by genre, a list of characters that make recurring appearances in fiction series, and a list of lesbian and feminist publishing houses.

These science fiction works frequently address the issue of feminist/lesbian separatist communities. See Lesbian science fiction for a more detailed review.

Fanfiction writers have produced many works in which female characters from fictional sources (such as television shows, movies, video games, anime, manga or comic books) are paired in romantic, spiritual, or sexual relationships. The genre is known by a variety of terms, including femslash, saffic, yuri and f/f slash. Lesbian content in fanfiction dates at least to 1977, but has become more popular during the 1990s and 2000s.