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Isabella MacDuff, Countess of Buchan (probably died c. 1314), was a significant figure in the Wars of Scottish Independence.

She was the daughter of Donnchadh III, Earl of Fife, and Johanna de Clare, daughter of The 6th Earl of Hertford. She was married to John Comyn, 3rd Earl of Buchan, and thus was the Countess of Buchan. After Robert the Bruce killed John III Comyn, Lord of Badenoch, at Greyfriars Kirk in Dumfries, the Earl of Buchan joined the English side in the Scottish Wars of Independence. Isabella took the contrary view.

According to tradition, the ceremony of crowning the monarch was performed by a representative of Clan MacDuff, but Isabella, Lady Buchan, arrived in Scone the day after the coronation of Robert the Bruce in March 1306. However, the Bruce agreed to be crowned for a second time the day after, as otherwise some would see the ceremony as irregular, not being performed by a MacDuff.