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The du Pont family (English: ) or Du Pont family is a prominent American family descended from Pierre Samuel du Pont de Nemours (1739–1817). It has been one of the richest families in the United States since the mid-19th century, when it founded its fortune in the gunpowder business. In the late 19th and early 20th centuries, it expanded its wealth through the chemical industry and the automotive industry, with substantial interests in the DuPont company, General Motors, and various other corporations.

Several former du Pont family estates are open to the public as museums, gardens or parks, such as Winterthur, Nemours, Eleutherian Mills, Longwood Gardens, Gibraltar, Mt. Cuba, and Goodstay. The family's interest in horticulture was planted in the United States by their immigrant progenitors from France and was also nourished and cultivated in later generations by avid gardeners who married into the family. As early as 1924, the du Ponts were recognized by Charles Sprague Sargent, the famed plantsman and director of Harvard's Arnold Arboretum, as "a family which has made the neighborhood of Wilmington, Delaware one of the chief centers of horticulture in the United States."