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Vocaloid 2 is a singing voice synthesizer and the successor to the Vocaloid voice synthesizer application by Yamaha. Unlike the first engine, Vocaloid 2 based its output on vocal samples, rather than voice analysis. The synthesis engine and the user interface were completely revamped, with Japanese Vocaloids possessing a Japanese interface, as opposed to the previous version, which used English for both versions. It is noteworthy for introducing the popular character Hatsune Miku.

Vocaloid 2 was announced in 2007. Unlike the first engine, Vocaloid 2 based its synthesis on vocal samples, rather than analysis of the human voice. Due to time constraints, unlike the previous engine, it did not have a public beta test and instead bugs were patched as users reported them. The synthesis engine and the user interface were completely revamped, with Japanese Vocaloids possessing a Japanese interface, as opposed to all versions being in english, as was the case with its predecessor. New features included note auditioning, transparent control track, toggling between playback and rendering, and expression control. One's breath and voice could also be recorded into the library to make realistic sounds. This version was not backward compatible with the original Vocaloid engine, and its editor could not load libraries built for the previous version.